Optimization of a Tricalcium Phosphate-Based Bone Model Using Cell-Sheet Technology to Simulate Bone Disorders

Bone diseases such as osteoporosis, delayed or impaired bone healing, and osteoarthritis still represent a social, financial, personal burden for affected patients society. Fully humanized in vitro 3D models of cancellous tissue are needed to develop new treatment strategies meet patient-specific needs. Here, we demonstrate successful cell-sheet-based process optimized mesenchymal stromal cell (MSC) seeding on β-tricalcium phosphate (TCP) scaffold generate tissue. Therefore, seeded MSCs onto the β-TCP scaffold, induced osteogenic differentiation, wrapped single osteogenically MSC sheet around pre-seeded scaffold. Comparing with an unwrapped did not detect any differences viability structural integrity but higher rate osteoid-like granular structures, indicator enhanced calcification. Finally, gene expression analysis showed reduction chondrogenic adipogenic markers, increase markers scaffolds. We conclude from these data that additional wrapping scaffolds will provide local niche enhances differentiation while repressing differentiation. This approach eventually lead preclinical strategies.